FRUCTOSE PRODUCTION FROM GLUCOSE BY ENZYMATIC METHOD

Document Type : Article

Authors

Bioenvironmental Research Center Sharif University of Technology

Abstract

Glucose syrup containing glucose and fructose was converted to fructose using glucose isomerase under optimized conditions, such as pH and temperature. Date syrup has sucrose, glucose and fructose. The main purpose of this research work is to convert the glucose of date syrup to fructose by enzymatic process and to obtain the optimum conditions for the best activity of the enzyme (IGI). This operation has been done in the same way as the process for producing HFCS. The process has been done in a batch system with immobilized glucose isomerase in specified ranges of pH: 6-8.5 and temp. (30-90)0C. The best optimum temp. and pH were (50-60)0C and 7-7.7, respectively, the required time would be 30 minutes.

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